Rapid Detection of Fungal Keratitis with DNA-Stabilizing FTA Filter Paper
نویسندگان
چکیده
منابع مشابه
Detection of fungi by conventional methods and semi-nested PCR in patients with presumed fungal keratitis
Background and Purpose: Fungal keratitis is a suppurative, ulcerative, and sight-threatening infection of the cornea that sometimes leads to blindness. The aims of this study were: recuperating facilities for laboratory diagnosis, determining the causative microorganisms, and comparing conventional laboratory diagnostic tools and semi-nested PCR. Materials and Methods: Sampling was conducted in...
متن کاملFungal Keratitis Associated with Viral Keratitis
Microbial keratitis caused by more than one microorganism is rare. It may occur as a coinfection or as a secondary infection superimposed with an existent microorganism. [1] Both infectious and immune mechanisms are implicated in microbial keratitis. Herein, we report an unusual clinical case of viral and fungal mixed infection. Written informed consent was obtained from the patient. A 31‑year‑...
متن کاملPneumocystis jiroveci in HIV/AIDS patients: detection by FTA filter paper together with PCR in noninvasive induced sputum specimens.
OBJECTIVES To detect P. jiroveci (previously named P. carinii) by PCR using FTA filter paper to extract the DNA, from noninvasive induced sputum samples of HIV/AIDS patients. MATERIAL AND METHOD Fifty two HIV/AIDS patients suspected of Pneumocystis jiroveci pneumonia (PJP) in King Chulalongkorn Memorial Hospital were recruited. Both cytological method and PCR with FTA filter paper technique w...
متن کاملFungal keratitis
CLINICAL QUESTION What is the most appropriate management of fungal keratitis? RESULTS Traditionally, topical Natamycin is the most commonly used medication for filamentous fungi while Amphotericin B is most commonly used for yeast. Voriconazole is rapidly becoming the drug of choice for all fungal keratitis because of its wide spectrum of coverage and increased penetration into the cornea. ...
متن کاملFungal keratitis.
PURPOSE OF REVIEW Throughout the world, fungal keratitis is a leading cause of ocular morbidity. The purpose of this review is to discuss the recently published literature in relation to the epidemiology, etiology, diagnosis, and therapy of fungal keratitis. RECENT FINDINGS Globally, the incidence of keratomycoses and systemic mycoses is rising. Current therapies are often ineffective. Ongoin...
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ژورنال
عنوان ژورنال: Investigative Opthalmology & Visual Science
سال: 2010
ISSN: 1552-5783
DOI: 10.1167/iovs.09-3737